Application

Western blot: 1-10 µg/mL (Chemiluminescence technique): NET can exist is at least two forms, a highly glycosylated ~80kDa form is the most common in brain, and a lessly glycosylated 54kDa form is also seen in some regions and tissues.



ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide/well) Optimal working dilutions must be determined by the end user.

This Anti-Norepinephrine Transporter Antibody is validated for use in ELISA, WB for the detection of Norepinephrine Transporter.

Immunogen

A 22 amino acid peptide sequence mapping to the 1st extracellular domain of rat NET.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA .

Specificity

Specific for Norepinephrine Transporter (NET). Norepinephrine Transporter is a 617 amino acid long integral membrane protein belonging to the Sodium:Neurotransmitter Symporter family (Bruss et al. 1995; Melikian et al. 1994; Pacholczyk et al. 1991) The immunogen peptide shows no significant homology with other other known proteins.

Target description

80 & 54 kDa